Explore the step-by-step process for staining mononuclear cells using fluorochrome-conjugated monoclonal antibodies specific for cell surface antigens.
Find procedures for whole blood specimen collection and preparation, including step-by-step instructions for lysing and staining. Use this method to detect cells bearing specific membrane antigens.
Explore four protocols using different methods (detergent method, mild or harsh alcohol method or harsh detergent method) for the activation of cell signaling molecules in mouse splenocytes or thymocytes.
Find instructions for immunofluorescent staining procedure and staining tips for mouse and rat leukocytes.
The indirect method is used to enhance the fluorescence signal and also to facilitate multicolor staining of human cells when direct conjugated reagents are not available. Find the procedure for specimen collection, preparation and staining of mononuclear cells.
Explore four protocols using different methods (detergent method, mild or harsh alcohol method or harsh detergent method) for the activation of cell signaling molecules in human peripheral blood mononuclear cells.
Explore four protocols using different methods (detergent method, mild or harsh alcohol method or harsh detergent method) for the activation of cell signaling molecules in human whole blood samples.